Journal: PLoS ONE

Article Title: Identification of Human Islet Amyloid Polypeptide as a BACE2 Substrate

doi: 10.1371/journal.pone.0147254

Figure Lengend Snippet: (A) RNAseq FPKM values for basal expression of human IAPP , BACE2 , BACE1 , PCSK1 , PCSK2 and APP in HEK293 cells. (B) HEK293 cells co-transfected with, or without, hIAPP plasmid DNA (1μg) and varying concentrations of hBACE2 plasmid DNA, hBACE1 plasmid DNA, or empty pCMV DNA, the latter to normalize DNA concentrations across conditions. A commercial source of Myc-DDK tagged hIAPP lysate (5 μg, last lane) was used to confirm identification of the hIAPP bands. The relative intensity of the top hIAPP band (C) and bottom hIAPP band (D) for each condition, as detected by anti-DDK and normalized to β-actin. Results are averaged from two separate experiments and the standard deviation is shown. (E) HEK293 cells co-transfected with hAPP, hBACE2, hBACE1 or empty pCMV DNA.

Article Snippet: Human IAPP, BACE2 and BACE1 proteins were detected using an anti-DDK monoclonal antibody (Origene).

Techniques: Expressing, Transfection, Plasmid Preparation, Standard Deviation